Table 3. Characteristics of dengue virus RNA positive patients.
Sample ID | Collection place and date | Travel history | Symptoms (days) | Patient age/ sex* |
Sampling day** | IgG (IFA) titre |
IgM (IFA) |
IgM EIA dengue |
Pan- flavi virus RT-PCR | DENV NS1 Ag | Virus isolation | Sequence confirmation |
---|---|---|---|---|---|---|---|---|---|---|---|---|
76 | Wundanyi 16-May-2016 |
Nairobi | Fever (2) |
3 F | 2 | <20 | <20 | Neg | Pos | ND | ND | DENV-2, partial cds NS5 132 bp (Sanger) *** |
85A | Wundanyi 10-May-2016 |
Mombasa | Fever (2) Joint pain (2) Myalgia (2) |
16 F | 2 | 640 | 20 | Neg | Pos | Pos | Pos | DENV-2, complete cds (NGS from virus isolate) GenBank accession: MK473385 |
c96 | Wundanyi 4-May-2016 |
Mombasa | Fever (2) Joint pain (2) Myalgia (2) Headache (2) |
19 F | 2 | <20 | <20 | Neg | Pos | Pos | Pos | DENV-2 complete cds (NGS from virus isolate) GenBank accession: MK473384 |
509 | Mwatate 24-Jun-2016 |
No recent travelling | Fever (3) Myalgia (3) Rash (3) |
46 M | 3 | <20 | <20 | Neg | Pos | Neg | Neg | DENV-2, partial cds, E-gene, 1485 bp Sanger GenBank accession: MK473383 |
222 | Voi 4-May-2016 |
Mombasa | Fever (2) | 17 M | 2 | 40 | <20 | Neg | Pos | Pos | ND | DENV-2, complete cds. direct NGS from sample, GenBank accession: MK473386 |
* M = male, F = female
** sampling day since onset of fever *** available from the authors upon request; DENV-2 = Dengue virus type 2