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. 2020 Mar 13;15(3):e0229502. doi: 10.1371/journal.pone.0229502

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© 2020 Yoon et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Background correction using the retrieved background method performs favourably in measurement of the absorbance spectra of nigrosin and methylene blue (a) Photograph of tissue-mimicking phantoms with intralipid (control), nigrosin (absorbing dye), and methylene blue (fluorescent dye). (b) Experimentally measured reflectance signals of the control phantom (intralipid only) at two different working distances gave the ground-truth background. (c) Absorbance of low and high concentrations of nigrosin dye calculated using the ground truth background (GT), single background (SB) and our retrieved background (RB) method respectively. (d) Absorbance of low and high concentrations of methylene blue calculated using GT, SB and RB methods respectively.