Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6JRj | Janvier Labs | ||
| Genetic reagent (Mus musculus) | Wnt1Cre | PMID: 9843687 | MGI:2386570 | Dr A Pierani (Imagine Institute) |
| Genetic reagent (Mus musculus) | Pax3Cre | PMID: 15882581 | MGI: 3573783 | Dr F Relaix (Créteil University) |
| Genetic reagent (Mus musculus) | Rosa26mTmG | PMID: 17868096 | MGI: 3716464 | Dr F Relaix (Créteil University) |
| Genetic reagent (Mus musculus) | DullardFlox | PMID: 23360989 | other | Dr R Nishinakamura (Kumamoto University) |
| Antibody | Anti-GFP (Chicken) | Aves Labs | GFP-1020 RRID:AB_10000240 |
IF(1:500) |
| Antibody | anti-PECAM (Rat monoclonal) | Santa-Cruz Biotechnology | Sc-18916 RRID:AB_627028 |
IF(1:200) |
| Antibody | Anti-phosphoSmad1/5/8 (Rabbit monoclonal) | Cell Signalling Technology | 13820S RRID:AB_2493181 |
IF (1:500) |
| Antibody | Anti-Myosin Heavy Chain (mouse monoclonal) | DSHB | MF20 RRID:AB_2147781 |
IF (1:300) |
| Antibody | Anti-phospho-histone H3 (rabbit polyclonal) | Cell Signalling Technology | 9701 RRID:AB_331535 |
IF (1:500) |
| Antibody | Anti-cleaved Caspase 3 (Rabbit monoclonal) | Cell Signalling Technology | 9664 RRID:AB_2070042 |
IF (1:500) |
| Antibody | Anti-Isl1 (Rabbit polyclonal) | Abcam | Ab20670 RRID:AB_881306 |
IF (1:500) |
| Antibody | AF 488 donkey IgG anti-chicken IGG (H+L) | Interchim | 703-545-155 RRID:AB_2340375 |
IF (1:500) |
| Antibody | Alexa Fluor 555 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed | Life Technologies | A-21429 RRID:AB_2535850 |
IF (1:500) |
| Antibody | AF 647 Donkey IgG Anti Rabbit IGG (H+L) | Interchim | 711-605-152 RRID:AB_2492288 |
IF (1:500) |
| Antibody | Alexa Fluor 647 Goat Anti-Rat IgG (H+L) | Life Technologies | A21247 RRID:AB_141778 |
IF (1:500) |
| Antibody | Alexa Fluor 647 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed | Life Technologies | A-21236 RRID:AB_2535805 |
IF (1:500) |
| Antibody | Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed | Life Technologies | A-11029 RRID:AB_2534088 |
IF (1:500) |
| Antibody | Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) Antibody | Life Technologies | A-11008 RRID:AB_143165 |
IF (1:500) |
| Antibody | Anti-Phospho Smad 2/3 (Rabbit monoclonal) | Ozyme | 8828 s RRID:AB_2631089 |
IF (1:200) |
| Sequence-based reagent | Sema3C | PMID: 16397144 | other | RNA probe from Dr S Zaffran (Aix Marseille University) |
| Sequence-based reagent | Dullard | Advanced Cell Diagnostics | #456911 | RNA probe |
| Sequence-based reagent | Sema3C | Advanced Cell Diagnostics | #441441 | RNA probe |
| Sequence-based reagent | Twist1 | Advanced Cell Diagnostics | #414701 | RNA probe |
| Sequence-based reagent | Snai2 | Advanced Cell Diagnostics | #451191 | RNA probe |
| Sequence-based reagent | Msx2 | Advanced Cell Diagnostics | #421851 | RNA probe |
| Sequence-based reagent | Id2 | Advanced Cell Diagnostics | #445871 | RNA probe |
| Antibody | Anti-Ter119 Pe-Cy7 (Mouse monoclonal) | Sony | 1181110 | Facs (1:300) |
| Antibody | Anti-CD45 APC-Cy7 (Mouse monoclonal) | BD Pharmingen | 557659 RRID:AB_396774 |
Facs (1:300) |
| Antibody | Anti-CD31 APC (Mouse monoclonal) | BD Pharmingen | Clone MEC 13.3 Catalog No. 561814 RRID:AB_10893351 |
Facs (1:300) |
| Commercial assay or kit | 7AAD PE-Cy7 | BD Pharmingen | 559925 | Facs (1:800) |
| Commercial assay, kit | CellsDirect One-Step qRTPCR Kit | Invitrogen | 11753100 | |
| Commercial assay, kit | 48.48 Sample/Loading Kit— 10 IFCs | Fluidigm Corporation | BMK-M10- 48.48 | |
| Cell Line (Mus musculus) | C2C12 | ATCC, PMID: 28966089 | CRL-1772, RRID:CVCL_0188 | |
| Peptide, recombinant protein | Human BMP-2 | Thermo Fisher Scientific | #PHC7145 | 50 ng/ml |
| Software, algorithm | R, pHeatmap (v1.0.10) | R foundation | R package (v3.2.2), RRID:SCR_016418 | |
| Software, algorithm | R, phenograph (v0.99.1) | R foundation | R package (v3.2.2), RRID:SCR_016919 | |
| Software, algorithm | R, ggplot2 (v3.1.0) | R foundation | R package (v3.2.2), RRID:SCR_014601 | |
| Software, algorithm | R, Destiny (v2.6.1) | R foundation | R package (v3.6) | https://github.com/theislab/destiny |
| Recombinant DNA reagent | pEGFP-Dullard (plasmid) | This paper | GFP-Dullard expression plasmid | |
| Recombinant DNA reagent | pEGFP-Dullard D67E (plasmid) | This paper | GFP-Dullard D67E expression plasmid | |
| Recombinant DNA reagent | pEGFP-N1 | Clontech | 6085–1 | |
| Commercial assay or kit | Gateway LR Clonase | ThermoFisher | 11791100 | |
| Commercial assay or kit | Taq Polymerase, Superscript III | Thermofisher | 11732020 | |
| Sequence-based reagent | Dull_FL_C1_FW | This paper | PCR primers | GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGATGCGGACGCAGTGT |
| Sequence-based reagent | Dull_FL_C1_Rev | This paper | PCR primers | GGGGACCACTTTGTACAAGAAAGTGGGTCTCACCAGAGCCTATGTTGGTG |
| Sequence-based reagent | Dull_D67E_FW | This paper | PCR primers | GATCCTGGTGCTGGAACTGGACGAAACCCTG |
| Sequence-based reagent | Dull_D67E_Rev | This paper | PCR primers | CAGGGTTTCGTCCAGTTCCAGCACCAGGATC |