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. 2020 Mar 13;9:e53129. doi: 10.7554/eLife.53129

Figure 5. SuM glutamate transmission is necessary for spatial memory retrieval.

Figure 5.

(A) Schematic diagram of genetic knockdown of Vgat or Vglut2 in SuM neurons. (B–C) Knocking down Vglut2 (B) but not Vgat (C) decreased discrimination ratio in the NPR test. (B, unpaired t-test, t14 = 3.662, **p = 0.0028; C, unpaired t-test, t14 = 0.000, p > 0.05). (D–E) Knocking down Vgat (D) or Vglut2 (E) did not alter the discrimination ratio in the NOR test, as compared to the control mice (D, n = 8 mice, unpaired t-test, t14 = 0.3378, p > 0.05; E, n = 8 mice, unpaired t-test, t14 = 0.066, p > 0.05). (F) Diagram of the experimental paradigm with the combination of in vivo optogenetic activation of SuM-DG projections and genetic knockdown of Vgat or Vglut2. (G–H) Knocking down Vglut2 (G), but not Vgat (H) impaired spatial memory retrieval upon optogenetic activation of SuM-DG projections (G, unpaired t-test, t20 = 3.888, **p = 0.0009; H, unpaired t-test, t16 = 1.935, p = 0.8327), as compared to the control mice injected with AAVs expressing shControl.