Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Ptprs | 19280 in ncbi | For iteration of next studies. | |
| Strain, strain background (Mus musculus; C57BL/6J) | Emx1-Cre;Ptprsfl/fl;Ptprs–l– mice | ES-Cell from KOMP: Ptrpstm1a(KOMP)Mbp | RRID: MGI_5797751 | gKO/cKO mice used in this study |
| Genetic reagent (Mus musculus) | Emx1-Cre | JAX | #005628 | Cre-expressing line used in this study |
| Transfected virus (Mus musculus) | pAAV1-hSyn-Cre-eGFP | Addgene | #105540 | Experimental virus for Cre injection (Figure 2 and Figure 6) |
| Transfected virus (Mus musculus) | pAAV1-hSyn-ΔCre-eGFP | Addgene | #105539 | Control virus for Cre injection (Figure 2 and Figure 6) |
| Transfected construct (Mus musculus) | pAAV-nEFCas9 | Addgene | #87115 | Vector for WT/Mut Ptprs expression (Figure 3). |
| Transfected construct (Mus musculus) | Ptprs WT gene | Li et al., 2015 | Ptprs(meA-/meB-) | Detailed sequence is added in Supplementary file 1. This used for mutagenesis and WT Ptprs rescue injection |
| Transfected construct (Mus musculus) | pAV-pU6-shGluN1#1-GFP | Vigene | #SH836303 | Experimental virus for Cre injection (Figure 6) |
| Transfected construct (Mus musculus) | pAV-pU6-shscrambled-GFP | Vigene | #SH836303 | Control virus for Cre injection (Figure 6) |
| Comparative phosphor-proteomic analysis | PhosphoSCAN service | Cell Signalling Technology | Phospho-tyrosine(pY-1000) | Used in Figure 4 |
| Antibody | anti-PTPσ (Guinea Pig polyclonal) | This paper | #2135 for N-term epitope, #2138 for C-term epitope | WB(1:500) |
| Antibody | anti-PSD93/SynGAP1/NGL3/SALM5/GluA1); (Rabbit,mouse polyclonal) | Home-made; used in previous studies from our group. | #1634(PSD93);#1682(SynGAP1);#2020(NGL3)#1943(SALM50;#1193(GluA1) | WB(1:500) |
| Antibody | anti-tubulin (mouse monoclonal) | DSHB | 12G10 | WB(1:5000) |
| Antibody | anti-GluN1(mouse))/GluN2A(rabbit)) monoclonal | Millipore | Mab363(GluN1);07-632(GluN2A) | WB(1:500) |
| Antibody | anti-GluN2B(mouse)/PSD95(mouse) monoclonal | NeuromAb | 73-101(GluN2B);75-028(PSD95) | WB(1:500) |
| Antibody | anti-Shank3(Rabbit;poly)/Synaptophysin(Mouse;mono) | Santa Cruz | H160(Shank3);D4(Synaptophysin) | WB(1:500) |
| Antibody | anti-linprinα3/caskin1 (Rabbit polyclonal) | Synaptic Systems | 169 102(Liprin α3); 185 003(caskin1) | WB(1:500) |
| Antibody | anti-liprinα2(Rabbit)/Trio(Mouse) polyclonal | Abcam | Ab155411(liprinα2);194365(Trio) | WB(1:500) |
| Antibody | anti-βcatenin(Mouse monoclonal) | BD Science | 610154 | WB(1:500) |
| Antibody | anti-N-Cadherin (Mouse monoclonal) | Thermo | 33–3900 | WB(1:500) |
| Antibody | anti-TrkC (Rabbit monoclonal) | Cell Signaling Technology | 3376 | WB(1:500) |
| Software, algorithm | GraphPad Prism 7.0 | GraphPad | Ver 7.0 | Used for all statistics used in the current study. |
| Software, algorithm | DAVID analysis | David.ncifcrf.gov | DAVID analysis | Used for statistics in proteomics |