Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomyces cerevisiae) | hsp82 |
Saccharomyces
Genome Database |
SGD: S000006161 | Hsp90 chaperone |
| Antibody | anti-Hsp90 α/β (Mouse monoclonal) | Cayman chemical; Cat# 10011439 | RRID:AB_10349777 | WB (1:3000) |
| Recombinant DNA reagent | Barcoded Hsp90 plasmid library | This paper | See Materials and methods for library construction | |
| Recombinant DNA reagent | p414ADH Δter plasmid | PMID: 23825969 | ||
| Recombinant DNA reagent | p414GPD plasmid | PMID: 23825969 | ||
| Commercial assay or kit | BCA Protein Assay Kit | Pierce | Cat #23227 | |
| Commercial assay or kit | KAPA SYBR FAST qPCR Master Mix | Kapa Biosystems | KK4600 | |
| Chemical compound, drug | Diamide | Sigma Aldrich | D3648 | |
| Software, algorithm | Barcode – Hsp90 ORF assembly | This paper | https://github.com/JuliaFlynn/Barcode_ORF_assembly | Associates barcodes with open reading frame mutations from paired end sequencing data (Flynn, 2020a; copy archived at https://github.com/elifesciences-publications/Barcode_ORF_assembly) |
| Software, algorithm | Tabulate Hsp90 counts | This paper | https://github.com/JuliaFlynn/Tabulate_counts | Counts Hsp90 alleles from raw fastq files and barcode_orf assembly file (Flynn, 2020b; copy archived at https://github.com/JuliaFlynn/Tabulate_counts) |
| Software, algorithm | EmpiricIST | PMID: 30127529 | https://github.com/Matu2083/empiricIST | Estimates selection coefficients based on the MCMC approach |
| Sequenced-based reagent | Sequencing primers | This paper | See Supplementary file 1 | |
| Sequenced-based reagent | Site-directed mutagenesis primers | This paper | See Supplementary file 1 | |
| Sequenced-based reagent | Library construction oligomers | This paper | Available upon request |