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. Author manuscript; available in PMC: 2020 Mar 13.
Published in final edited form as: Nat Chem Biol. 2018 Dec 10;15(2):115–122. doi: 10.1038/s41589-018-0181-6

Figure 1. Design of the proteolysis-based signaling pathways and orthogonal proteases.

Figure 1

(a) Scheme of components of the proteolysis-based signaling pathways. (b) Heat map showing orthogonality of the four potyviral protease homologues tested in HEK293T cells, detected by the cycLuc reporter with a matching protease cleavage site. (c,d) Chemically inducible reconstitution of the split proteases 24h after induction with rapamycin (three-dimensional homology models of orthogonal split proteases are shown in Supplementary Fig. 1e). Values in (d) are the mean of four cell cultures ± (s.d.) and are representative of two independent experiments. Transfection plasmid mixtures are listed in Supplementary Table 1.