Extended Data Fig. 10. EM averages and reconstructions.
(a) Representative subset of single particle nsEM 2D class averages (left) and corresponding reconstructed 3D maps (right). The 3D reconstructions are an average composite of most particles in each dataset and reveal the epitopes targeted in each serum sample as labeled. (b) Per animal epitope analysis showing a single segmented Fab per epitope as determined by further 3D classification of average composite reconstructions shown in Supplementary Figure 7. Colors represent the region of trimer where Fabs bind (blue - V5, pink - N355/alpha2 helix, purple - base, and yellow - N611). Three separate rabbit sera were analyzed for both MD39 and pSer8-MD39 immunizations.