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. 2020 Mar 12;40(3):BSR20193315. doi: 10.1042/BSR20193315

Figure 2. Agomir-miR-129-5p improves recovery of SCI mice by reducing apoptosis.

Figure 2

The mice were subjected to SCI and then treated with agomir-miR-129-5p/agomir-NC (50 μl/day, 100 nmol/ml) for 3 days (0, 1, and 2 days) via intrathecal injection starting 15 min after contusion SCI. At indicated time, the animals were killed and subsequently, a 10-mm long segment of the spinal cord was harvested for further experiments. (A) qRT-PCR was performed to determine the expression levels of miR-129-5p in spinal cord tissues at 1, 3, 7, 14, and 28 days after agomir-129-5p injection. (B) The BBB scores at 1, 3, 7, 14, 21, and 28 days after SCI were shown for all groups of mice. (C) Cresyl Violet staining was used to assess spared tissue following behavioral analyses at 28 days post-injury. (D) Spinal cord water content was assessed using wet-to-dry weight method. (E) IHC analysis of cleaved caspase-3 at 7 days post-injury in spinal cord tissues. (F) The protein expressions of Bcl-2, Bax, cleaved caspase-3 and cleaved PARP were measured by Western blot. Data represent the mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. Sham group; ##P<0.01 vs. SCI + agomir-NC group.