Fig. 2.

Insulin fibrillation at different pH values. a Kinetics of insulin amyloid formation monitored by ThT fluorescence in citrate–phosphate buffers with indicated pH. The initial insulin monomer concentration was 64 μM. b Normalized ThT emission from (a), including 64 µM insulin aggregated in ammonium acetate buffer with indicated pH. All experiments were performed in triplicate, all replicates are shown in the plot