Fig. 1. Workflow of the integrated MS-based approach for characterizing the light-driven modulation of paired PSII–LHCIIsc.

Isolation of heterogeneous mixtures of PSII–LHCIIsc from pea plants grown at different light intensities (L, low; C, moderate used as control; H, high). PSII–LHCIIsc preparations were used for TD-MS and XL-MS in vitro, performed with DSSO and EDC crosslinkers on two independently isolated PSII–LHCIIsc for each light condition pooled together. DSSO XL-MS in situ was conducted on thylakoid membranes isolated from three independent batches of pea plants grown in moderate (C) light.