Table 1.

Energy intake, anthropometric measures at tissue collection and plasma measures.

Measure	Chow diet		Cafeteria diet		p Values
	Vehicle	Minocycline	Vehicle	Minocycline	Diet	Mino	Interaction
Total energy and macronutrient intake (average kJ/rat)
Total energy intake	14432 ± 282	15292 ± 989	45584 ± 2628a,b	41886 ± 719a,b	<0.001	–	–
Total protein intake	3140 ± 67	3269 ± 211	3768 ± 150a	3355 ± 64	0.023	–	–
Total carbohydrate intake	9419 ± 200	9806 ± 632	25292 ± 1407a,b	23574 ± 334a,b	<0.001	–	–
Total fat intake	1952 ± 41	2032 ± 131	13400 ± 719a,b	11998 ± 373a,b	<0.001	–	–
Anthropometric measures (cm)
Naso-anal length	23.8 ± 0.3	24.6 ± 0.3	25.2 ± 0.2a	24.9 ± 0.2a	0.002	–	0.034
Girth	17.7 ± 0.2	18.1 ± 0.1	20.5 ± 0.3a	19.9 ± 0.4a	<0.001	–	–
Tibia length	4.10 ± 0.03	4.10 ± 0.06	4.17 ± 0.04	4.12 ± 0.04	–	–	–
Organ weights (g)
Liver weight	14.3 ± 0.4	15.4 ± 0.6	22.3 ± 1.4a,b	23.0 ± 1.4a,b	<0.001	–	–
Heart weight	1.04 ± 0.04	1.12 ± 0.03	1.38 ± 0.04a,b	1.29 ± 0.11a,b	<0.001	–	0.035
Fat pad weights (g)
Retroperitoneal	4.03 ± 0.26	3.48 ± 0.23	14.67 ± 1.53a,b	12.66 ± 1.15a,b	<0.001	–	–
Gonadal	4.23 ± 0.35	3.94 ± 0.27	14.62 ± 1.47a,b	12.04 ± 1.31a,b	<0.001	–	–
Total	8.27 ± 0.55	7.43 ± 0.47	29.28 ± 2.86a,b	24.70 ± 2.26a,b	<0.001	–	–
Unfasted blood and plasma measures
Blood glucose (mmol/L)	9.5 ± 0.4	9.8 ± 0.4	10.2 ± 0.4	10.6 ± 0.4	–	–	–
Plasma insulin (ng/mL)	1.55 ± 0.15	1.16 ± 0.05	2.62 ± 0.43a,b	1.85 ± 0.21	0.002	0.034	–
Plasma leptin (ng/mL)	3.65 ± 0.30	3.35 ± 0.28	10.65 ± 1.75a,b	12.39 ± 1.44a,b	<0.001	–	–
Plasma triglyceride (mmol/L)	0.86 ± 0.09	0.75 ± 0.07	2.84 ± 0.28a,b	2.14 ± 0.22a,b	0.034	<0.001	–
Plasma high-density lipoprotein (mmol/L)	0.56 ± 0.03	0.46 ± 0.03	0.45 ± 0.04	0.50 ± 0.04	–	–	0.026

Data expressed as mean ± SEM; n = 4 cages for energy intake measures; n = 10–12 for other measures. Data were analyzed using two-way ANOVA, followed by post hoc multiple comparisons with a Tukey HSD correction.

^ap < 0.05 relative to CVeh.

^bp < 0.05 relative to CMino.

^cp < 0.05 relative to CafVeh.