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. 2020 Mar 13;11:1383. doi: 10.1038/s41467-020-15031-3

Fig. 2. ERK5i induce transcriptional activity in the ERK5:MEF2D reporter system independently of kinase activity.

Fig. 2

a, b, c and e HEK293 cells were transfected with GAL4-MEF2D, GAL4:LUC and CMV:Renilla, together with either wild-type HA-ERK5 (full length) or HA-ERK5 ΔTAD and EGFP-MEK5D or EGFP (control) as indicated. Four hours post transfection, cells were treated with either DMSO (control) or compound 26 (a), compound 25 (b), AX15836 (c) or BIX02189 (e) at concentrations indicated. Twenty-four post transfection, cells were lysed and firefly luciferase activity was measured and normalised to Renilla. The results are presented as the mean of three independent experiments ± SEM. Source data are provided as a Source Data file. d HEK293 cells were transfected as in (a). Twenty-four hours post transfection cells were lysed and processed as in a. f HEK293 cells were transfected with GAL4-MEF2D, GAL4:LUC and CMV:Renilla together with either wild-type HA-ERK5 (full length) or HA-ERK5 kinase dead and EGFP-MEK5D or EGFP (control) as indicated. Twenty-four hours post transfection cells were lysed and processed as in a. g and h HEK293 cells were transfected as in (f). Four hours post transfection, cells were treated with either DMSO (control) or compound 26 (g) or AX15836 (h) at concentrations indicated. Twenty-four hours post transfection cells were lysed and processed as in (a).