Table 1.
Antibodies and conditions used.
| Antibody | Supplier | Catalogue number | Dilution/ diluent | Technique |
|---|---|---|---|---|
| Anti-MEK5 | EMD Millipore | AB3184 | 1:500/5% milk TBS-T | Immuno-blotting |
| Anti-phospho-TEY ERK5 | Cell Signaling | 3371 | 1:500 / 5% BSA TBS-T | Immuno-blotting |
| Anti-FLAG M2 | Roche/Sigma | F3165 | 1:2000 / 5% milk TBS-T | Immuno-blotting |
| Anti-HA (12CA5) | Sigma | 11583816001 | 5 µg/ml in Antibody Dilution Buffer: (1x PBS/1% BSA/0.3% Triton™ X-100) | Immuno-fluorescence |
| Anti-HA | Babraham Institute Monoclonal Facility | N/A | 1:10/5% milk TBS-T | Immuno-blotting |
| Anti-phospho-T733 | Gift from Dr. Atanasio Pandiella, University of Salamanca, Spain. | N/A | 1:250/5% milk TBS-T | Immuno-blotting |
| Anti-phospho-S754 | Gift from Dr. Atanasio Pandiella, University of Salamanca, Spain. | N/A | 1:250/5% milk TBS-T | Immuno-blotting |
| Anti-myc (9E10) | Santa Cruz | SC-40 | 1:1000/5% milk TBS-T | Immuno-blotting |
| Anti-phospho-T359 RSK | Cell Signaling | 8753 | 1:1000/5% milk TBS-T | Immuno-blotting |
| Anti-RSK | Cell Signaling | 9355 | 1:1000/5% milk TBS-T | Immuno-blotting |
| Anti-MEK1/2 | Cell Signaling | 9122 | 1:1000/5% milk TBS-T | Immuno-blotting |
| Anti-Lamin A/C | Santa Cruz | SC-7292 | 1:500/5% milk TBS-T | Immuno-blotting |
| Anti-ERK5 (C-7) | Santa Cruz | SC-398015 | 1:1000/5% milk TBS-T | Immuno-blotting |