Figure 8.

Growth of CRC cells after incubation with bacterial supernatants. TA was incubated with bacteria (Sgg + TA, Sii + TA) or buffer (TA), for 24 h to reduce the TA concentration in the solution. Bacteria without TA (Sgg, Sii) were used as negative control. Cells treated with buffer alone were used as control. Bacterial supernatants were sterilized by filtration and added to three different tumor cell lines at final estimated concentration of 100 µM TA. Quantitative analysis of adherent cells was done by crystal violet staining after 72 h of incubation. Results show data of three independent experiments. Mean ± SD; *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001; one way ANOVA (Holm-Šídák test).