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. 2020 Mar 13;3:121. doi: 10.1038/s42003-020-0854-z

Fig. 6. Elevation of an ER stress marker and decrease of myelin marker in L-MPZ mice.

Fig. 6

a Immunoblotting of sciatic nerve homogenates (5 µg per lane) in 10-week-old L-MPZ mice using an ER stress marker (anti-GRP78), non-myelinated Schwann cell marker (anti-GFAP), and myelin marker (anti-MBP) antibodies. GAPDH was used as a loading control. M molecular weight marker. b–d Quantification of band intensities refer to a. L-MPZ homozygous (Hom) mice showed an increased level of GRP78 (b) and GFAP (c) levels and decrease of MBP (d) in the sciatic nerve. e, f qPCR analysis of sciatic nerves in L-MPZ mice. Levels of P0 (e) and MBP (f) mRNA were normalized to HPRT mRNA levels. mRNA levels of MBP and P0 were decreased in L-MPZ Hom mice. *p < 0.05; ***p < 0.001 by ANOVA with post-hoc Tukey’s test. Data are presented as mean ± SE of four experiments. N (mice) in WT = 4, Het = 6, Hom = 4 (b–d); and WT = 4, Het = 4, Hom = 4 (e, f). Uncropped blots for a can be found in Supplementary Fig. 10.