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. 2020 Mar 13;10(3):38. doi: 10.1038/s41408-020-0305-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Bone marrow B1 B-cell progenitor analysis of 2-month-old mice. Upper panel: representative plots of FACS analysis of B1 (Lin−IgM−CD93+B220−CD19+) B-cell progenitor. Lower panel: percentage and absolute cell numbers of the bone marrow Lin−IgM−CD93+B220−CD19+ population. Each symbol represents one mouse. Nfkbie^+/+ (n = 5), Nfkbie^+/− mice (n = 6) and Nfkbie^−/− mice (n = 6). b Analysis of total B cells (CD19+), B1 (CD19+B220low) and B2 (CD19+B220+) subset distribution in the spleen and peritoneal cavity. Each symbol represents one mouse. Nfkbie^+/+ (n = 5), Nfkbie^+/− mice (n = 6), and Nfkbie^−/− mice (n = 6). c Analysis of splenic transitional (T1 (CD19+B220+CD93+IgMhiCD23−) and T2 (CD19+B220+CD93+IgMhiCD23+)) B cells, MZB cells and FoB cells distribution. Each symbol represents one mouse. Nfkbie^+/+ (n = 5), Nfkbie^+/− mice (n = 6), and Nfkbie^−/− mice (n = 6). Data are mean ± SEM. *p < 0.05, **p < 0.01; ns, not significant.