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. 2020 Mar 13;10(3):38. doi: 10.1038/s41408-020-0305-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Peripheral blood was monitored for the presence of CD19+B220low B cells starting at 4 months. Each symbol represents one mouse (n = 10). b Right panel: percentage of CD5+ cells among CD19+B220low population in the peripheral blood of 16-month-old mice. Each symbol represents one mouse. Left panel: representative plots of FACS analysis of the CD19+B220low B cells and of the expression of CD5 on the CD19+B220low population in the peripheral blood of Nfkbie^+/+ (n = 10), Nfkbie^−/− without MBL(−/−) (n = 6), and Nfkbie^−/− with MBL (−/−MBL) (n = 4). c Analyses of BCR rearrangement clonality in sorted Nfkbie^+/+ CD19+B220+ B cells, and Nfkbie^−/− CD19+B220+ and CD19+B220low B cells of two different 14-month-old mice (#1 and #2). V–J junctions were PCR amplified from cDNA of sorted B cells using VH family primers (VH1 and multiplexed PCR of VH2, VH3, VH5, VH6, and VH7) and FAM-conjugated consensus JH primer to assess the IgH CDR3 diversity. d Spleen weight of old (>12 months) Nfkbie^+/+ (n = 13) and Nfkbie^−/− mice (n = 14). Each symbol represents one mouse. e Representative image from littermate Nfkbie^+/+ and Nfkbie^−/− old mice showing enlarged inguinal lymph nodes of Nfkbie^−/− mice. f Representative flow cytometry profiles of CD19+B220low infiltration in bone marrow, spleen, lymph nodes, thymus, and peritoneal cavity of 18-month-old Nfkbie^−/− mice with MBL (n = 5) and age-matched Nfkbie^+/+ mice. g Absolute cell numbers of CD19+B220low B cells in the spleen and peritoneal cavity of old Nfkbie^+/+ and Nfkbie^−/− mice (n = 11). Each symbol represents one mouse. Data are mean ± SEM. *p < 0.05, **p < 0.01.