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. 2020 Mar 13;10:4703. doi: 10.1038/s41598-020-61586-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of CNT-DOX-Fe₃O₄-Tf and CNT modifications to obtain the multicomponent CNT-DOX-Fe₃O₄-Tf (nanobot). (A) TEM microscopy images of CNT-DOX-Fe₃O₄-Tf, (B) evidencing Fe₃O₄ structure, and (C) crystalline features of the NPs. (D) FTIR spectra of of (a) CNT-COOH, (b) CNT-DOX, (c) CNT-DOX-Fe₃O₄ and (d) CNT-DOX-Fe₃O₄-Tf. (E) surface charge evolution upon loading of CNT with DOX and further conjuagtion of Fe₃O₄ and Tf, (F) UV-visible spectra of DOX (λ_max = 480 nm), Tf (λ_max = 280 nm) and CNT-DOX-Fe₃O₄-Tf (Tf peak at 280 nm and DOX peak at 480 nm). (G) Normalized fluorescence spectra of DOX and CNT-DOX-Fe₃O₄-Tf (λ_ex = 480 nm, λ_em = 590 nm).