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. 2020 Mar 13;11:1370. doi: 10.1038/s41467-020-15081-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The heatmap shows the collection of AMR genes identified in 15 members of the pBT2436-like megaplasmid family through BLASTn searches against the Comprehensive Antibiotic Resistance Database (CARD)⁵⁶. Percentage of coverage of the AMR genes identified from the searches is indicated. The megaplasmids (Y axis) are hierarchically clustered based on their content of AMR genes (X axis) using the “complete” method with Euclidean distance⁵⁷. The “_N” suffix in gene names denotes cases where the gene occurs more than once in the same megaplasmid. AMR genes are additionally classified based on the drug class they confer resistance to according to CARD⁵⁶.