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. 2020 Mar 13;11(3):183. doi: 10.1038/s41419-020-2384-5

Fig. 1. ZnO NPs trigger oxidative stress-dependent, iron-mediated ferroptosis.

Fig. 1

HUVECs were treated with ZnO NPs (0, 5, and 10 μg/mL) for 24 h. a GSH, b GPx, c ROS, and d MDA levels were measured. Total RNA was extracted for the analysis of mRNA levels of interest using qRT-PCR. e GPx4, f ACSL4, g ALOX15, and h PTGS2 mRNA. HUVECs were pretreated with DFO (100 μM) for 1 h in the presence of 10 μg/mL ZnO NPs. i The generation of ROS was determined by the DCFH-DA probe, MFI: mean fluorescence intensity. j Cell viability analysis. k LDH leakage assay. Data are shown as the mean ± S.D. from three independent experiments.