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. 2020 Mar 13;10:4696. doi: 10.1038/s41598-020-61756-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil Elastase 680 and acriflavine CLE with severity scoring. (A) Representative CLE video frames for different disease severity features. Acriflavine signal in green; NE680 signal in red. (B) Scores determined by video analysis with mean scores plotted. For DSS, n = 10; for DSS + IL22Fc, n = 10; for naive, n = 10. Values presented are the mean ± S.D. One-way ANOVA was used to analyze variation; ***P < 0.001.