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. 2020 Mar 13;10:4696. doi: 10.1038/s41598-020-61756-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil Elastase 680 ex vivo colon imaging. (A) Resected mouse colon highlighting different levels of neutrophil activity by treatment group. (B) Whole organ ROI analysis show decrease in NE680 signal for DSS + IL22Fc treated animals. (C,D) Segmented analysis of distal and proximal colon. For DSS, n = 20; for DSS + IL22Fc, n = 20; for naive, n = 20. All values presented are the mean ± S.D. One-way ANOVA was used to analyze variation; ***P < 0.001.