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. 2020 Mar 13;11(3):186. doi: 10.1038/s41419-020-2367-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Experiments were performed to validate hCSCs using company reported markers. a Immunofluorescence staining for Sca1+, MEF2C, and NKX2.5. Western blotting for MEF2C and Nkx2.5. β-actin was used as a loading control. Lane 1, 2, and 3 are three different samples. b Immunofluorescence of cardiomyocyte marker β-MHC. It is a negative control showing absence of differentiated hCSCs.