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. 2020 Mar 13;11(3):186. doi: 10.1038/s41419-020-2367-6

Fig. 5. Ablation of MMP9 prevents hyperglycemia-induced pyroptosis in hCSCs.

Fig. 5

Both hCSCs and MMP9−/− hCSCs were incubated with normal (NG) or hyperglycemic (HG) culture medium for 24 h. These treated cells were used for evaluation of pyroptosis markers. a, b, d, e. Western blotting and densitometric analyses of NLRP-3, ASC, caspase-1, and gasdermine D (GSDMD) proteins. β-actin is a loading control. c, f Immunofluorescence and quantification of intensity of cellular levels of ASC and IL-1β protein. g Cell viability evaluation by ATP levels. Increased ATP indicates increased cell viability. Cells were treated with HG or NG in the presence of JNK inhibitor/vehicle. Each dot represents one sample. Values are expressed as mean ± SEM. One-way ANOVA and Tukey’s post-hoc test was used. P < 0.05 is considered statistically significant.