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. 2020 Mar 13;10:4625. doi: 10.1038/s41598-020-61490-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of co-activators Yap1 and Taz affects cell fate, neuronal migration and cortical layering. (a) Experimental paradigm used to perform overexpression of Yap1 and Taz, and GFP as a control. IUEs were performed at E13.5 and brains isolated at and analyzed at E15.5, after 48 hours. (b) Illustration to show the sequential generation of distinct types of cortical layers, specified by different TFs. The cortical development is divided in expansion, neurogenesis and gliogenesis. (c) Coronal sections of transfected cortices immunostained for GFP and Tbr1, deep layer marker. (d) Quantification of Tbr1⁺GFP⁺ cells shows a reduction upon overexpression of Yap1 and Taz, compared to GFP control in CP and total. (e) Coronal sections of transfected cortices immunostained for GFP and Ctip2, deep layer marker. (f) Quantification of Ctip2⁺GFP⁺ cells shows a reduction compared to GFP control in CP and no change in total, upon overexpression of Yap1 and Taz. IUE- in utero electroporation. Total = VZ+SVZ/IZ+CP. Also see Fig. S3. Summaries of the quantifications are in Table S1. Scale bar = 50 μm. Data are shown as average ± SEM, *p = 0.05, **p = 0.01, ***p = 0.001.