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. 2020 Mar 13;10(3):37. doi: 10.1038/s41408-020-0304-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — P-gp⁺ and CD34⁺ MP counts in CD138⁻ and CD138⁺ MP populations were determined using flow cytometry. a A sequential gating strategy using MP size (left panel) followed by CD41a expression (middle panel) and CD138 expression (right panel) was applied to the total MP population (left panel). The total MP population (CD41a⁻) was defined based on ± staining for anti-CD41a (middle panel). b The total MP population was gated based on CD138 expression. We phenotyped for the presence of CD138⁻ P-gp⁺ CD34⁺ MPs (left panel, gate P1) and CD138⁺ P-gp⁺ CD34⁺ MPs (right panel, gate P4). c The CD138⁻ P-gp⁺ CD34⁺ and CD138⁺ P-gp⁺ CD34⁺ MP subpopulations (gates P1 and P4 of left and right panel in b, respectively) were gated and phenotyped for PS⁺ events using annexin V (left panel, gate P11; right panel, gate P12), respectively.