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. 2020 Mar 13;10:4662. doi: 10.1038/s41598-020-61688-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Copy number alterations (CNAs) and EGFR gene expression levels detected in primary tumors from patients with metastatic and non-metastatic sCRC. CNA status assessed by Affymetrix 500‐K single nucleotide polymorphism (SNP)‐array platform (panel A) and FISH techniques [probes for identifying chromosome 7 centromere (7p11; green spots) and EGFR gene (7p11.2; red spots)] (panel B). EGFR gene expression profile analyzed by oligonucleotide arrays (Affymetrix PrimeView Human Gene Expression microarray) (panel C) and immunostaining techniques (20x) (panel D). Panel (B,D) correspond to a sample of the same patient.