Table 3.
Clinical, biological and immunohistochemical characteristics of sporadic colorectal cancer patients (n = 50) and their association with overall survival.
| N | Univariate analysis | Multivariate analysis | |||
|---|---|---|---|---|---|
| HR (95% CI) | p-value | HR (95% CI) | p-value | ||
| Variable | |||||
| Age | |||||
| <72 years | 28 (55%) | Ref. | 0.7 | ||
| ≥72 years | 23 (45%) | 1.14 (0.56–2.31) | |||
| Gender | |||||
| Male | 38 (76%) | Ref. | 0.9 | ||
| Female | 13 (25%) | 0.94 (0.40–2.18) | |||
| Site of primary tumor | |||||
| Colon | 37 (73%) | Ref. | 0.8 | ||
| Rectum | 14 (27%) | 1.13 (0.48–2.66) | |||
| TNM stage at diagnosis | |||||
| StageI/II | 27 (53%) | Ref. | <0.001* | Ref. | |
| Stage III | 4 (8%) | 2.33 (1.29–4.21) | 3.20 (0.67–15.14) | 0.143 | |
| Stage IV | 19 (39%) | 2.17 (1.60–2.94) | 58.37 (8.66–393.62) | <0.001* | |
| Tumor size | |||||
| <4 cm | 10 (20%) | Ref. | 0.9 | ||
| ≥4 cm | 41 (80%) | 1.05 (0.43–2.57) | |||
| CEA serum levels | |||||
| <5 ng | 21 (48%) | Ref. | 0.008* | Ref. | 0.188 |
| ≥5 ng | 23 (52%) | 2.81 (1.28–6.16) | 0.38 (0.09–1.61) | ||
| Microsatellite instability | |||||
| No | 42 (84%) | Ref. | 0.8 | ||
| Yes | 8 (16%) | 1.12 (0.48–2.62) | |||
| EGFR | |||||
| <35% | 36 (73%) | Ref. | 0.005* | Ref. | 0.044* |
| ≥35% | 13 (27%) | 3.15 (1.36–7.33) | 0.31 (0.10–0.97) | ||
| β-catenin | |||||
| Negative | 29 (58%) | Ref. | 0.6 | ||
| Positive | 21 (42%) | 0.82 (0.39–1.72) | |||
| CEA | |||||
| Negative | 11 (22%) | Ref. | 1 | ||
| Positive | 39 (78%) | 0.99 (0.45–2.16) | |||
| c-Myc | |||||
| <10% | 9 (19%) | Ref. | 0.6 | ||
| ≥10% | 39 (81%) | 0.80 (0.32–1.96) | |||
| Her2 | |||||
| Negative | 49 (98%) | NA | NA | ||
| 3+ | 1 (2%) | ||||
| Ki-67 | |||||
| <50% | 34 (69%) | Ref. | 1 | ||
| ≥50% | 15 (31%) | 1.00 (0.45–2.20) | |||
| p53 | |||||
| 0 | 13 (26%) | Ref. | 0.7 | ||
| <10% | 15 (30%) | 1.36 (0.53–3.48) | |||
| ≥10% | 22 (44%) | 0.99 (0.61–1.59) | |||
Results expressed as number of cases (percentage). HR: hazard ratio. Ref.: reference group for HR calculation. *Statistically significant at p < 0.05. NA: not available, too few data elements in one group.
Stats for multivariant model: Concordance = 0.803 (se = 0.057); R2 = 0.495; Likelihood ratio test = 28.67 (p < 0.001); Wald test = 26.02 (p < 0.001); Score test = 37.55 (p < 0.001).