Table 3.

Oligonucleotide real-time qPCR primers

Gene	Accession number1	Primer sequence (5′ → 3′)	Orientation	Product size (bp)
IL-1β	NM_204524	CGAGGAGCAGGGACTTTGC GAAGGTGACGGGCTCAAAAA	Forward Reverse	71
IL-18	GU119895	TGCAGCTCCAAGGCTTTTAAG CTCAAAGGCCAAGAACATTCCT	Forward Reverse	63
TNFα	NM_204267	CGTTTGGGAGTGGGCTTTAA GCTGATGGCAGAGGCAGAA	Forward Reverse	61
NLRP3	XM_001233261	GTTGGGCAGTTTCACAGGAATAG GCCGCCTGGTCATACAGTGT	Forward Reverse	63
CRP	NM_001039564	AAGCTCAGGACAACGAGATCCT TTTCCCCCCCACGTAGAAG	Forward Reverse	71
HSP60	NM_001012916	CGCAGACATGCTCCGTTTG TCTGGACACCGGCCTGAT	Forward Reverse	55
HSP70	J02579	GGGAGAGGGTTGGGCTAGAG TTGCCTCCTGCCCAATCA	Forward Reverse	55
OCLN	NM_205128	CGCAGATGTCCAGCGGTTA GTAGGCCTGGCTGCACATG	Forward Reverse	59
CLDN1	NM_001013611	CCCACGTTTTCCCCTGAAA GCCAGCCTCACCAGTGTTG	Forward Reverse	61
18S	AF173612	TCCCCTCCCGTTACTTGGAT GCGCTCGTCGGCATGTA	Forward Reverse	60

¹Accession number refers to Genbank (NCBI). CLDN1, claudin 1; CRP, C-reactive protein; HSP, heat shock protein; IL, interleukin; NLRP3, nucleotide-binding oligomerization domain leucine-rich repeat and pyrin domain containing 3; OCLN, occluding; TNFα, tumor necrosis factor alpha.