Figure 5.

Mutagenesis and computational modelling reveal different residues contribute to NAM cooperativity. (a) Ronacaleret, (b) ATF936, and (c) Pfizer compound 1 are shown in sticks and balls docked in the 7TM domain (white helical ribbons). Mutated residues are shown as sticks and are coloured according to a greater than twofold increase (light teal), greater than fivefold increase (dark blue), or greater than fivefold decrease (orange) in cooperativity. While residues that contribute to BMS compound 1 affinity are shown (d), BMS compound 1 is not docked in the 7TM because predicted docking poses were not compatible with the mutagenesis or SAR data