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. 2020 Feb 13;25(4):816. doi: 10.3390/molecules25040816

Figure 3.

Figure 3

Flow cytometry analysis of mode of cell death in colicin N-treated lung cancer cells (a) Annexin V-FITC/propidium iodide (PI) histograms were obtained from analysis of lung cancer H460 cells incubated with 5–15 µM of colicin N for 24 h. Treatment with colicin N significantly increased apoptosis indicating with annexin V-FITC positive cells. (b) The decrease of %living cells corresponding with the augmentation of early and late apoptosis was observed in colicin N-treated H460 cells. Notably, flow cytometry showed minor alteration of necrosis cells after treatment the cells with colicin N. Representative experiment out of three is shown.