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. 2020 Jan 24;9(2):100. doi: 10.3390/antiox9020100

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Percentages of viable spermatozoa showing high superoxide (O₂^-•) levels (E⁺/YO-PRO-1⁻), (B) mean E⁺ fluorescence intensity of viable spermatozoa showing high superoxide levels, (C) percentages of viable spermatozoa showing high peroxide (H₂O₂) levels (DCF⁺/PI⁻), and (D) mean DCF⁺ fluorescence intensity of viable spermatozoa showing high peroxide levels of semen samples treated with ethacrynic acid (EA), a glutathione S-transferases (GSTs) inhibitor, and the control group, assessed at different evaluation times during liquid storage at 17 °C (0, 24, 48, and 72 h). Different letters (a, b) indicate significant differences (p < 0.05) between treatments within storage time.