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Immunolocalisation of sperm GSTM3. (A,B) control group at 0 h of storage at 17 °C, (C,D) control group at 72 h of liquid-storage, (E,F) Ethacrynic acid (EA)-treated spermatozoa at 72 h of liquid-storage, (G) negative control, and (H) peptide competition assay. White arrows indicate the sperm midpiece. The nucleus is shown in blue colour (DAPI), whereas GSTM3 is shown in green (fluorescein isothiocyanate, FITC). Scale bars: A, C, E, H: 30 μm; D, G: 15 μm; B, F: 10 μm.
