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. 2020 Feb 11;25(4):773. doi: 10.3390/molecules25040773

Figure 5.

Figure 5

Inhibition effects of compound 6c (0 μM, 2.5 μM, 5 μM, and 10 μM) on fibrogenic markers, TGF-β1/Smads pathway and JAK1/STAT3 pathway. (A) The protein expression levels of COL1A1, α-SMA, MMP2, TGF-β1, p-Smad2/3, and Smad2/3 in LX-2 cells after treatment with the indicated concentrations of 6c together with TGF-β1 (2 ng/mL) for 24 h were detected by western blot assay. (B) The protein expression levels of p-JAK1, JAK1, p-STAT3, STAT3, p-STAT1, STAT1 in LX-2 cells after treatment with the indicated concentrations of 6c together with TGF-β1(2 ng/mL) for 24 h were detected by western blot assay. GAPDH served as internal reference. (C–G) Quantification of the intensities of COL1A1 (C), α-SMA (D), MMP2 (E), and TGF-β1 (F) of (A). (H–J) Quantification of the intensities of p-JAK1 (H), p-STAT3 (I), and p-STAT1 (J) of (B). Data were presented as the mean ± SEM, # p < 0.05, ## p < 0.01 as compared to that of control group; * p < 0.05, ** p < 0.01 as compared to that of TGF-β1 group.