Table 1.

List of mutant oncogenes detected with PNA clamped nucleic acid amplification. Accompanying methods used for detection are described in column 2. Limits of detection are listed in column 3 (N/A means the information was Not Available). Length of the PNA used is listed in column 4 (kit refers to a PNA that was part of a kit and the PNA length was not described). References are listed in column 5.

Oncogene	Method Used in Combination with PNA Clamped PCR	Mutated DNA Detected in Presence of Wild-Type DNA	PNA Sequence Length- Number of Nucleobases	Refs
KRAS	DNA Sequencing	0.5%	15	[59]
	Fluorescent Probes	0.03%	17	[60]
	Melting Curve Analysis	0.05%, 0.001%	15,17	[61,62]
	Fluorescent PNA Sensor with LightCycler	0.01%	17	[63]
	High Fidelity DNA Polymerase	0.005%	17	[64]
	SmartAmp2	1%	17	[65]
	Asymmetric PCR	0.1%	17	[66]
	Modified PNA Detection Probes	1%	17	[67]
	Next Generation Sequencing	N/A	6	[70]
p53	PCR-SSCP	N/A,0.1%,0.05%	15,15,15	[71,72,73]
EGFR	PNA+LNA	0.1%	14-18	[75]
	DNA Sequencing	0.1%	Kit	[77]
	Fluorescent Melting Curve Analysis	0.1%,N/A,0.01%	Kit	[78,79,80]