Table 2.

Genes, forward and reverse primers for gene expression analysis by qRT-PCR.

Gene 1	5’-Primer Sequence Forward- 3´	5´-Primer Sequence Reverse- 3´	Ref.
UB	GGGATGCAGATCTTCGTGAAA	CTTGCCAGCAAAGATCAACCTT	[25]
β Actin	GTGATGGACTCTGGTGATGG	TGGTGAAGCTGTAGCCTCTC	[26]
TLR-2β	CGCTTAGGAGAGACAATCTGTGAA	GCCTGTTTTAGGGATTTCAGAGAATTT	[27]
TLR4	AGTCTGAAATTGCTGAGCTCAAAT	GCGACGTTAAGCCATGGAAG	[27]
TGF-β4	CGGCCGACGATGAGTGGCTC	CGGGGCCCATCTCACAGGGA	[27]
IL-8 (CXCLi2)	CCTGGTTTCAGCTGCTCTGT	GCGTCAGCTTCACATCTTGA	[28]
Bu-1	GGTGTCCAGTGAAGGTGTG	GATGCAAAGGATGGGTGTC	[29]
HSP-70	GGCTGGAGAGAAGAATGTGC	CAGCTGTGGACTTCACCTCA	[30]
Claudin1	TGGCCACGTCATGGTATGG	AACGGGTGTGAAAGGGTCATAG	[27]
Claudin3	GCCAAGATCACCATCGTCTC	CACCAGCGGGTTGTAGAAAT	[30]
FABP6	TGATTTCCCTGGACTCAGC	CCCACCTTCCATTTTGACTG	[31]
ChREBP	CTGAGCGATCGAAGGTGAA	TCTCCATCTTGCTGGAGTCA	[32]

¹ UB, ubiquitin; TLR4, toll-like receptor 4; TLR-2β, toll-like receptor 2β; TGF-β4, transforming growth factor beta 4; IL-8, interleukin 8 (former CXCLi2); Bu-1, chicken B-cell marker chB6; HSP-70, heat shock protein 70; Claudin1, Claudin-1; Claudin3, Claudin-3; FABP6, fatty acid binding protein 6; ChREBP, carbohydrate-responsive element-binding protein.