Figure 2.

Intra- and inter-day precision of the (A) apolipoprotein and (B) adipokine multiple reaction monitoring (MRM)-based assay. One pooled isotope-labeled peptide standard mixture of all targeted apolipoproteins and adipokines was divided into three aliquots, respectively. One aliquot was directly injected into the mass spectrometer (MS) (day 0) to evaluate the intra-day precision. The other two aliquots were stored at −80 °C and subjected to the MS at day 3 and day 6, respectively. Each analysis was performed in technical quintuplicates. The coefficient of variation (CV) values are displayed in percentages for all targeted 10 apolipoproteins and 9 adipokines. Scatter plots show mean ± SD (standard deviation).