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. 2020 Jan 22;12(2):295. doi: 10.3390/nu12020295

Figure 3.

Figure 3

Effect of Borlotti bean extract on (a) cell viability and (b–d) pro-inflammatory cytokine expression in macrophages. (a) Murine RAW264.7 macrophages were incubated with 50, 100 and 200 μg/mL as well as 5 μM sulforaphane (SFN) and DMSO (1%) and cell viability was assessed by neutral red assay. Negative control (NC) refers to DMEM medium only. (b) Effect of Borlotti bean extracts on mRNA inflammatory target genes IL6 (b), IL1-β (c) and iNOS (d). Macrophages were incubated with 50 (B50), 100 (B100) and 200 μg/mL (B200) cooked Borlotti bean extract and stimulated with LPS (100 ng/mL). Controls used were unstimulated medium control (DMEM), LPS-stimulated control (LPS) and sulforaphane (5 μM) control (SFN). * indicates statistically different values among samples according to a post-hoc comparison (Dunnett’s test) at p < 0.05.