Table 1.
Primers used for PCR
| Target gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Mouse primer sequences | ||
| Adgre1 | TCTGCAGTGTCAGCTCAGAA | GAAGTCTGGGAATGGGAGCT |
| αSMA | CTGACAGAGGCACCACTGAA | CATCTCCAGAGTCCAGCACA |
| Bdnf | GGTATCCAAAGGCCAACTGA | CTTATGAATCGCCAGCCAAT |
| Ccl2/Mcp1 | CCAAATGAGATCAGAACCTACAACT | CTAGTTCACTGTCACACTGGTCACT |
| Cd31 | TGCAGGAGTCCTTCTCCACT | ACGGTTTGATTCCACTTTGC |
| Cldn5 | CTGGACCACAACATCGTGAC | GCCGGTCAAGGTAACAAAGA |
| Ctnnb1 | AGGGTGCTATTCCACGACTA | CACCCTTCTACTATCTCCTCCAT |
| Desmin | GACATCCGGGCTCAGTATGA | CGCGCAATGTTGTCCTGATA |
| Fgf2 | GGCTGCTGGCTTCTAAGTGT | CCGTTTTGGATCCGAGTTTA |
| Fn1 | AATGGAAAAGGGGAATGGAC | CTCGGTTGTCCTTCTTGCTC |
| Gfap | AAGGTTGAATCGCTGGAGGA | AAGGTTGAATCGCTGGAGGA |
| Igf1 | TGGATGCTCTTCAGTTCGTG | GTCTTGGGCATGTCAGTGTG |
| Mag | TGCTCACCAGCATCCTCACG | AGCAGCCTCCTCTCAGATCC |
| Mbp | TACCTGGCCACAGCAAGTAC | GTCACAATGTTCTTGAAG |
| Ntf3 | GATCCAGGCGGATATCTTGA | AGCGTCTCTGTTGCCGTAGT |
| Pdgfb | GGCCACACACCTTCTCTGAT | GTGGAGGAGCAGACTGAAGG |
| Pdgfrb | CACCTTCTCCAGTGTGCTGA | GGAGTCCATAGGGAGGAAGC |
| Plp | GTATAGGCAGTCTCTGCGCTGAT | AAGTGGCAGCAATCATGAAGG |
| Tnfa | CGTCAGCCGATTTGCTATCT | CGGACTCCGCAAAGTCTAAG |
| Zo1 | GGGCCATCTCAACTCCTGTA | AGAAGGGCTGACGGGTAAAT |
| 18s rRNA | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
| Human primer sequences | ||
| Ccl2/Mcp1 | GATCTCAGTGCAGAGGCTCG | TGCTTGTCCAGGTGGTCCAT |
| Il6 | GAACTCCTTCTCCACAAGCG | TTTTCTGCCAGTGCCTCTTT |
| Tnfa | CACTAAGAATTCAAACTGGGGC | GAGGAAGGCCTAAGGTCCAC |
| Pdgfrb | AGGTGGTTGCACATTTGTCCAG | TGTGCAGCTGTGTTCTTGAGAC |
| 18s rRNA | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |