Figure 1.
Carrier of Wingless (Cow) genetic locus and mutant characterization. A, Intron/exon structure of the cow gene (transcript cow-RD; flybase.org). Arrowheads indicate gene disruption project (gdp) inserts in two different lines (03259 and 12802). The third arrowhead indicates where the published cow5Δ deletion begins in the 3′ UTR and runs 9119 bp downstream (Chang and Sun, 2014). Below, the expanded region outlined with the red box is labeled “fwd” and “rev” to depict the RT-PCR primer pair. B, PCR products from the genotypes listed. Control (dfmr1 gene) and cow primers from the region of the cow5Δ deletion. C, D, RT-PCR products from the genotypes listed using both control (dmgalectin gene) and cow primers. E, Western blot of the indicated genotypes using an anti-Cow antibody, with the total protein stain shown below. The two arrows indicate Cow protein with and without GAG chains.