Figure 8.

Cow and Notum act in the same Wg pathway to limit NMJ bouton number. A, Confocal images of the muscle 4 NMJ colabeled with presynaptic HRP marker (green) and postsynaptic DLG marker (red) in the genetic background control (w¹¹¹⁸), cow null heterozygote (cow^GDP/+), notum null heterozygote (notum^KO/+), and cow/notum transheterozygote (cow^GDP/notum^KO). Quantified bouton number is shown to the right. B, High-magnification NMJ confocal images of anti-Wg labeling at synaptic boutons of the same indicated genotypes. The presynaptic HRP marker boundary is outlined in white. Quantified Wg fluorescence intensity is shown to the right, normalized to the background control (w¹¹¹⁸). C, Confocal images of the muscle 4 NMJ colabeled with presynaptic HRP marker (green) and postsynaptic DLG marker (red) in the genetic background control (w¹¹¹⁸), cow null (cow^GDP/cow^GDP), notum null (notum^KO/notum^KO), and cow/notum double null (cow^GDP,notum^KO/cow^GDP,notum^KO). Quantified bouton number is shown to the right. Data shown in scatter plots, with mean ± SEM. p Values are shown for each statistical comparison.