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. 2020 Feb 21;25(4):971. doi: 10.3390/molecules25040971

Figure 6.

Figure 6

K313 induces autophagy blockage in Nalm-6 and Daudi cells in a dose- and time-dependent manner. Different concentrations of K313, 5 μM rapamycin, and 20 μM hydroxychloroquine (HCQ) were used to treat (A) Nalm-6 and (B) Daudi cells for 24 h. Then, the whole cell lysates were analyzed by Western blotting using LC3 and P62 antibodies. Quantitative analyses of protein levels were conducted in (C) Nalm-6 and (D) Daudi cells. Further, 8 µM K313 was used to treat (E) Nalm-6 and (F) Daudi cells at different time-points, and the total cell lysates were analyzed by Western blot. (G) After incubation with or without 16 µM K313 for 24 h in MCF-7, Hela, and A549 cells, the whole protein lysates were tested by Western blot using LC3 and P62 protein antibodies. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control group.