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. 2020 Jan 21;12(2):285. doi: 10.3390/nu12020285

Table 2.

Experiment conditions and the extraction yields of the alkaloids for the Box–Behnken design.

Run Factors Extraction Yield (μg/g)
X1 (min) X2 (W) X3 (°C) Higenamine Liensinine Dauricine Isoliensinine Neferine
1 5 500 90 405.1 255.2 1372.8 15.9 1008.9
2 17.5 1500 120 874.7 856.5 3015.7 131.3 2424.6
3 17.5 1000 90 750.5 744.6 2450.6 56.7 2202.1
4 17.5 1000 90 762.3 554.9 2372.6 92.5 1991.5
5 30 1000 120 912.6 835.4 2413.5 106.7 2315.6
6 17.5 1000 90 935.4 761.9 3050.6 42.5 2776.5
7 17.5 500 120 677.9 667.0 2285.3 88.4 1808.9
8 5 1000 60 228.3 139.2 767.6 21.5 644.5
9 17.5 1500 60 205.4 136.7 771.3 20.3 632.4
10 30 500 90 582.3 536.6 2170.5 71.6 1611.1
11 5 1000 120 717.4 630.2 2395.1 88.7 1895.8
12 30 1000 60 122.0 50.8 529.0 33.6 458.4
13 30 1500 90 715.3 673.6 2549.0 84.7 2030.7
14 17.5 1000 90 707.8 684.9 2185.5 59.6 2169.2
15 5 1500 90 250.5 130.8 836.9 19.4 675.8
16 17.5 500 60 261.8 136.1 858.0 21.9 734.1
17 17.5 1000 90 756.5 757.7 2171.8 71.4 1933.0

X1: Extraction time; X2: Microwave power; X3: Temperature.