Table 2.
Multiple comparisons in contrast analysis. Post hoc analysis of glutathione reductase (GR), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), total superoxide dismutase (SOD), Mn-dependent superoxide dismutase (MnSOD), copper–zinc superoxide dismutase (CuZnSOD) activity and malondialdehyde (MDA) concentration in erythrocytes and heart muscle of rats from study groups subjected to high-fat (HF) and/or to (CD) control diets and subjected to DJOS (duodenal-jejunal omega switch) or SHAM (control) surgery. Statistical significance was set at p < 0.05. Statistically significant values are bolded.
| Oxidative Stress Markers Post hoc | DJOS vs. SHAM | DJOS | SHAM | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1: HF/HF | 2: HF/CD | 3: CD/HF | 4: CD/CD | 1 vs. 2 | 1 vs. 3 | 1 vs. 4 | 2 vs. 3 | 2 vs. 4 | 3 vs. 4 | 1 vs. 2 | 1 vs. 3 | 1 vs. 4 | 2 vs. 3 | 2 vs. 4 | 3 vs. 4 | |
| Erythrocytes | ||||||||||||||||
| GR (IU/g) | 0.189 | <0.001 | <0.001 | 0.557 | <0.001 | <0.05 | 0.207 | <0.001 | <0.001 | 0.226 | 0.114 | <0.001 | 0.594 | <0.001 | <0.05 | <0.001 |
| CAT (IU/g) | <0.001 | <0.05 | <0.001 | <0.05 | <0.001 | <0.001 | <0.001 | 0.056 | 0.375 | 0.259 | <0.01 | <0.001 | <0.01 | <0.001 | 0.490 | <0.001 |
| GPx (IU/g) | <0.001 | <0.01 | 0.939 | <0.05 | 0.324 | <0.001 | <0.001 | <0.01 | <0.001 | 0.180 | 0.771 | 0.542 | 0.094 | 0.758 | 0.178 | 0.296 |
| GST (IU/g) | <0.001 | 0.303 | <0.001 | 0.178 | <0.001 | <0.001 | <0.001 | 0.382 | <0.001 | <0.001 | 0.058 | <0.001 | 0.127 | <0.001 | <0.01 | <0.001 |
| Total SOD (NU/mg) | 0.876 | 0.763 | 0.857 | 0.542 | <0.01 | 0.181 | <0.001 | 0.115 | 0.293 | <0.01 | <0.01 | 0.156 | <0.01 | 0.091 | 0.898 | 0.070 |
| MDA (μmol/g) | <0.001 | 0.142 | 0.774 | <0.001 | <0.001 | <0.01 | <0.001 | 0.205 | <0.001 | <0.001 | 0.154 | 0.356 | <0.05 | 0.621 | <0.01 | <0.01 |
| Heart Muscle | ||||||||||||||||
| GR (IU/g) | <0.001 | <0.001 | <0.001 | 0.572 | <0.01 | <0.01 | <0.05 | 0.771 | 0.803 | 0.589 | <0.001 | <0.001 | <0.001 | 0.956 | <0.001 | <0.001 |
| CAT (IU/g) | <0.001 | 0.529 | 0.742 | 0.635 | <0.05 | 0.171 | 0.075 | 0.273 | 0.500 | 0.669 | <0.001 | <0.001 | <0.001 | 0.425 | 0.668 | 0.222 |
| GPx (IU/g) | 0.461 | <0.01 | 0.064 | 0.270 | <0.05 | 0.095 | 0.160 | 0.705 | 0.513 | 0.782 | 0.911 | 0.586 | 0.291 | 0.676 | 0.361 | 0.618 |
| Total SOD (NU/mg) | <0.001 | 0.300 | <0.05 | <0.01 | <0.001 | <0.05 | 0.192 | <0.05 | <0.01 | 0.280 | <0.05 | <0.001 | <0.05 | <0.001 | 0.650 | <0.001 |
| MnSOD (NU/mg) | <0.05 | 0.380 | 0.113 | 0.215 | <0.05 | 0.096 | 0.150 | 0.475 | 0.343 | 0.813 | <0.001 | <0.05 | <0.01 | 0.154 | 0.190 | 0.904 |
| CuZnSOD (NU/mg) | 0.222 | <0.05 | <0.01 | <0.001 | <0.001 | <0.05 | 0.277 | <0.05 | <0.001 | 0.188 | 0.116 | <0.05 | <0.001 | <0.001 | <0.001 | <0.001 |
| MDA (μmol/g) | <0.001 | <0.001 | <0.001 | 0.303 | 0.230 | <0.01 | 0.514 | <0.001 | 0.578 | <0.001 | <0.05 | <0.001 | <0.001 | <0.001 | <0.001 | 0.990 |
Column 1: Inter-group comparisons between study groups pre-HF diet vs. post-HF diet, pre-CD vs. post-HF diet, per-HF vs. post-CD and pre-CD vs. post-CD; groups DJOS vs. SHAM. Column 2: Intra-group comparisons between pre-HF diet vs. post-HF diet, pre-CD vs. post-HF diet, pre-HF vs. post-CD and pre-CD vs. post-CD groups after DJOS surgery. Column 3: Intra-group comparisons between pre-HF diet vs. post-HF diet, pre-CD vs. post-HF diet, pre-HF vs. post-CD and pre-CD vs. post-CD groups after SHAM surgery.