Figure 3.
The rat glomerular mesangial cells (GMCs) were pretreated with various concentrations of compounds 2, 4, 6, 7, and 9 (5, 10, 20, 40, and 80 μM) and incubated for 48 h. GMCs proliferation was determined using the 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Values are expressed as mean ± SD of three independent experiments, with # p < 0.01 relative to the 5.6 mM glucose (normal group, NG), and ** p < 0.01, * p < 0.05 relative to the 25 mM high glucose (high-glucose group, HG).
