Figure 2.

Stimulation of the translocation of hormone-sensitive lipase (HSL) and perilipin-1 by Rimonabant during adipocyte lipolysis. Primary rat adipocytes were incubated (20 min, 37 °C) in the absence or presence of increasing concentrations of Rimonabant. Subsequently, LD were prepared from the homogenate of the washed adipocytes by centrifugation through a sucrose cushion (see Materials and Methods). The top fractions were extracted for removal of lipids. Proteins were then separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed for HSL and perilipin-1 by immunoblotting. The images of a typical experiment with electrophoretic runs in duplicate and repeated two times are shown with similar results. hHSL, recombinant human HSL was run in parallel as marker. The quantitative evaluation of the three experiments is shown (mean ± SD). * p ≤ 0.05 vs. absence of Rimonabant.