Figure 5.

Activation of PKA by Rimonabant during adipocyte lipolysis. Primary rat adipocytes were incubated (37 °C) in the absence or presence of 1 µM isoproterenol (●) or 10 µM Rimonabant (■) for increasing periods of time and then rapidly deep-frozen. After preparation of cytosolic fractions, identical amounts of protein were assayed for the PKA activity ratio (see Materials and Methods). The portion of PKA activated at the time point of deep-freezing of the adipocytes was calculated as the ratio of Kemptide phosphorylated in the absence and presence of cAMP. Mean ± SD of three incubations with electrophoretic separations in duplicate. * (10 µM Rimonabant), # (1 µM Rimonabant) p ≤ 0.01 vs. time 0.