Figure 8.

Stimulation of lipolysis in a cell-free system by Rimonabant. LD prepared from untreated rat adipocytes, which had been metabolically labelled with NBD-FA, were reconstituted with partially purified rat adipocyte HSL (see Materials and Methods) and then incubated (30 °C) for increasing periods in the absence (control) or presence of Rimonabant. The organic phase of the chloroform/methanol/HCl-extract of the total incubation mixture was analyzed by thin layer chromatography (TLC). The amount of NBD-FA lipolytically released from the NBD-FA-labelled acylglycerols (AG) 1–4 and run in parallel to a synthetic NBD-FA marker (ma) was quantitatively evaluated by fluorescence imaging. A fluorogram of a typical experiment with TLC runs in duplicate repeated two times is shown with quantitative evaluation (mean ± SD) of three independent incubations with TLC analysis in duplicate. * p ≤ 0.05 for LD incubated between absence and presence of Rimonabant.