Figure 9.

Increase in the amount of LD-associated HSL in a cell-free system by Rimonabant. Primary rat adipocytes were incubated (20 min, 37 °C) in the absence or presence of increasing concentrations of Rimonabant. Subsequently, LD were prepared from the washed adipocytes, reconstituted with partially purified rat adipocyte HSL and then incubated (90 min, 30 °C). LD were recovered from the total incubation mixtures by centrifugation through a sucrose cushion from the top fraction 6 (see Materials and Methods). Extracted proteins were separated by SDS-PAGE and analyzed for HSL (■) and perilipin-1 (●) by immunoblotting. The images of a typical experiment are shown repeated two times with similar results. The quantitative evaluation of the three experiments is also shown (mean ± SD). * p ≤ 0.05 for HSL recovered with LD between absence and presence of Rimonabant; no significant differences for perilipin-1.